This medium is recommended for the detection, isolation and enumeration of yeasts and moulds. For mycological counts it may be desirable to prepare the more acid medium in order to suppress bacterial growth. The slide is double sided and the other side contains Nutrient Agar with TTC. A general purpose agar medium, containing two peptones, which will support the growth of a wide variety of organisms. It is suitable for the cultivation both of aerobes and anaerobes, the latter being grown either in deep cultures or by incubation under anaerobic conditions. A small quantity of the dye 2,3,5- triphenyltetrazolium (TTC) is added. Aerobic bacteria species grow on this medium and they can be detected by their ability to reduce TTC to a red colored formozan dye. Bacterial colonies appear as red dots on an otherwise clear colorless medium.