The immunoaffinity column can selectively adsorb Deoxynivalenol (Vomitoxin, DON) and 15-acetyldeoxynivalenol (15-ADON), and has a highly targeted purification effect. Samples that have passed through the column for purification may be used in HPLC, LC-MS/MS and other devices for analysis after concentrating with nitrogen gas.

Affinity columns can be used in combination with HPLC or LC-MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

DON belongs to the single-endomycin family of molds and is produced by certain Fusarium fungi. 15-ADON is derivative of DON. These toxins are mostly distributed in grain seeds such as wheat, barley, and corn. Due to their high cytotoxicity and immunosuppressive properties, they pose a health threat to humans and animals.

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the aflatoxin in the sample is extracted, filtered, and diluted, and then passed slowly through the aflatoxin immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Deoxynivalenol is then eluted with methanol and injected into an analytical instrument for detection.

Specifications: