The immunoaffinity column can selectively adsorb Ochratoxin A from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC analysis or for LC-MS/MS analysis after being concentrated with nitrogen gas and resuspended.

Affinity columns can be used in combination with HPLC to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

Ochratoxin A (OTA) is a toxic metabolite produced by some species of Aspergillus and Penicillium,it is a mycotoxin with high kidney and liver toxicities, and has teratogenic, mutagenic, and carcinogenic effects.

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the Ochratoxin A in the sample is extracted, filtered, and diluted, and then passed slowly through the Ochratoxin A immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Ochratoxin A is then eluted with methanol and injected into an analytical instrument for detection.

Specifications: