Easy DryTM are absorbent pads saturated with a sterile, dehydrated culture medium. Each pad is pre-plated in a Petri dish and is immediately ready to use after pouring sterile distilled or deionized water on it. Easy DryTM are optimal for the examination of large sample volumes by the membrane filter method.
Easy DryTM Bile Aesculin Azide is a selective medium used for the confirmation and enumeration of enterococci in water and foodstuffs.
This medium complies with the specification given by ISO 7899-2.
PREPARATION
1.
Cut open a bag and remove the number of Easy Dry plates needed.
2.
Moisten the pad contained
in the Petri dish with 2.2 ml of sterile distilled or deionized water.
3.
Wait 5 minutes before
using.
TEST PROCEDURE
Filter the sample through a filter membrane (0.45 µm pore diameter). Transfer the membrane onto a plate containing a just rehydrated pad of Easy Dry Slanetz Bartley (87508). Incubate aerobically at 36 ± 2°C for 40-48 hours. Typical colonies show a red, maroon or pink color, either in the centre or throughout the colony.
Confirm by transferring the membrane and the colonies onto a pad of Easy Dry Bile Aesculin Azide just rehydrated and pre-warmed to 44°C. Incubate at 44 ± 0.5°C for 2 hours.
INTERPRETING RESULTS
Count all typical colonies showing a brown to black color in the surrounding medium as intestinal enterococci.
APPEARANCE OF THE MEDIUM
Whitish pad. Yellowish once rehydrated.
STORAGE
Store at 10-25°C away from light. Do not
use the product beyond its expiry date on the label or if product shows any
evidence of contamination or any sign of deterioration.
SHELF LIFE
2 years.
