The immunoaffinity column can selectively adsorb zearalenone from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC or LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.

Immunoaffinity columns can be used in combination with HPLC/LC-MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

Zearalenone (ZEN), also known as F-2 toxin, is mainly produced by Fusarium strains. Its components are 2,4-dihydroxybenzoic acid lactones produced by Fusarium graminearum and Fusarium culmorum. ZEN mainly pollutes crops such as corn, wheat, and grains. It has a strong estrogenic effect and can cause hyperestrogen, as well as severe reproductive tract symptoms and infertility, and is also immunotoxin and genotoxic.

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and passed slowly through the zearalenone immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Zearalenone is eluted with methanol, and then injected into the analytical instrument for detection.

Specifications: