This Photometer for Reducing Sugars in Wine combines accuracy and ease of use in an ergonomic, portable design. A user can accurately determine the concentration of reducing sugars in wine within a 0.00 to 50.0 g/L (ppt) range using the HA-HI83746-20 ready-made reagents.
This device uses the Fehling method to determine the concentration of reducing sugars less than 50.00 g/L (ppt). When Fehling’s A and Fehling’s B Solutions react with a sample containing reducing sugars, the sample will undergo a color change; the greater the concentration, the deeper the color. The associated color change is then colorimetrically analyzed according to the Beer-Lambert Law.
This principle states that light is absorbed by a complementary color, and the emitted radiation is dependent upon concentration. For determination of reducing sugars, a narrow band interference filter at 610 nm (orange) allows only orange light to be detected by the silicon photodetector and omits all other visible light emitted from the tungsten lamp. As the change in color of the reacted sample increases, absorbance of the specific wavelength of light also increases, while transmittance decreases.
Typical content of reducing sugars in must and wine:
Must | sweet must normal in fermentation | 20 to 25 % 10 to 20 % 4 to 12.5 % | 200 to 250 g/L 100 to 200 g/L 40 to 125 g/L |
Wine | sweet semi-sweet almost dry dry | 2.5 to 12.5 % 0.8 to 2.5 % 0.2 to 0.8 % 0 to 0.2 % | 25 to 125 g/L 8 to 25 g/L 2 to 8 g/L 0 to 0.2 g/L |
Features:
Specifications:
Range | 0.00 to 50.00 g/L (ppt) |
Resolution | 0.25 g/L |
Precision | +/- 0.015 @ 0.350 g/L |
Method | Fehling |
Power Supply | 1.5V AA batteries (4) / 12 VDC adapter; auto-off after 15 minutes of non-use |
Environment | 0 to 50°C (32 to 122°F); RH max 95% non-condensing |
Ordering Information | HA-HI83746 is supplied with glass cuvettes and caps (4), reagents for approx. 20 tests (HA-HI83746-20), HA-HI93703-59 charcoal, 200 μL automatic pipette with two plastic tips, 1000 μL automatic pipette with plastic tips (2), instruction sheet for automatic pipette, spoon, funnel, filter paper (25), cuvette wiping cloth, 12 VDC adapter, batteries, instruction manual, instrument quality certificate, and rigid carrying case. |