Used for the detection and
quantitative determination of wild yeast populations in brewing culture yeast,
and especially non-Saccharomyces yeast.
LCSM is used for the detection and quantitative determination
of wild yeast populations in brewing culture yeast. It is designed to
encourage the growth of non-Saccharomyces yeast. An inoculum of approximately 1
million culture yeast cells needs to be plated on LCSM. While the growth
of culture yeast will be suppressed by cupric sulfate, wild yeast will develop
as large, distinct colonies. A few Saccharomyces, like S.
cerevisiae var. diastaticus, may be more resistant to cupric sulfate and show
a certain level of growth.
